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cell lines 369 primary human pulmonary microvascular endothelial cells hpmec  (PromoCell)


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    Structured Review

    PromoCell cell lines 369 primary human pulmonary microvascular endothelial cells hpmec
    Cell Lines 369 Primary Human Pulmonary Microvascular Endothelial Cells Hpmec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+pulmonary+microvascular+endothelial+cells+hpmecs/Human+Pulmonary+Microvascular+Endothelial+Cells/pm42262358-129-10-20
    Average 96 stars, based on 162 article reviews
    cell lines 369 primary human pulmonary microvascular endothelial cells hpmec - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Modeling the Effects of IL-1β-mediated Inflammation During Ex Vivo Lung Perfusion Using a Split Human Donor Model
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMECs) (PromoCell GmbH) were seeded onto the chips at 1.5 × 107/ml and left to attach for 15 min. Forty microliters of treatments were added to each well per channel with HPMECs then incubated at 37°C/5% CO2 for 4 h. For blocking experiments, perfusates were preincubated with an IL-1β neutralizing antibody (NAb) (R&D Systems) at 4 μg/ml for 30 min prior. ..

    Article Title: Modeling the Effects of IL-1β-mediated Inflammation During Ex Vivo Lung Perfusion Using a Split Human Donor Model
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMECs) (PromoCell GmbH) were seeded onto the chips at 1.5 × 10 7 /ml and left to attach for 15 min. Forty microliters of treatments were added to each well per channel with HPMECs then incubated at 37°C/5% CO 2 for 4 h. For blocking experiments, perfusates were preincubated with an IL-1β neutralizing antibody (NAb) (R&D Systems) at 4 μg/ml for 30 min prior. ..

    Blocking Assay:

    Article Title: Modeling the Effects of IL-1β-mediated Inflammation During Ex Vivo Lung Perfusion Using a Split Human Donor Model
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMECs) (PromoCell GmbH) were seeded onto the chips at 1.5 × 107/ml and left to attach for 15 min. Forty microliters of treatments were added to each well per channel with HPMECs then incubated at 37°C/5% CO2 for 4 h. For blocking experiments, perfusates were preincubated with an IL-1β neutralizing antibody (NAb) (R&D Systems) at 4 μg/ml for 30 min prior. ..

    Article Title: Modeling the Effects of IL-1β-mediated Inflammation During Ex Vivo Lung Perfusion Using a Split Human Donor Model
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMECs) (PromoCell GmbH) were seeded onto the chips at 1.5 × 10 7 /ml and left to attach for 15 min. Forty microliters of treatments were added to each well per channel with HPMECs then incubated at 37°C/5% CO 2 for 4 h. For blocking experiments, perfusates were preincubated with an IL-1β neutralizing antibody (NAb) (R&D Systems) at 4 μg/ml for 30 min prior. ..

    Cell Culture:

    Article Title: TRPV2 channels facilitate pulmonary endothelial barrier recovery after ROS-induced permeability
    Article Snippet: .. Cells 80 Primary human pulmonary microvascular endothelial cells (HPMECs) [29] from healthy 81 donors were obtained from Promocell (Heidelberg, Germany, #C-12281) and cultured in 82 endothelial cell growth medium MV (Promocell, #C-22020) at 37 °C and 5 % CO2, and were kept 83 until passage 12. ..

    Article Title: MiR-483-5p has the potential to serve as a biomarker in perioperative respiratory adverse events in children under general anesthesia
    Article Snippet: .. Human pulmonary microvascular endothelial cells (HPMECs) were provided by PromoCell (C-12281, Heidelberg, Germany) and cultured in endothelial cell medium (ECM) supplemented with FBS, antibiotics, and endothelial cell growth serum. ..

    Article Title: The Protein Kinase A Inhibitor KT5720 Prevents Endothelial Dysfunctions Induced by High-Dose Irradiation.
    Article Snippet: For flow cytometry analysis, all antibodies (BB515 mouse anti-human CD54 (ICAM-1, cat no. 564685), BUV737 mouse anti-human CD106 (VCAM-1, cat no. 565418), PE mouse anti-human CD62P (P-Selectin, cat no. 555524), APC mouse anti-human CD62E (ESelectin, cat no. 551144)), and the FITC Annexin V/Propidium Iodide apoptosis detection kit were obtained from BD Biosciences (Franklin Lakes, NJ, USA). .. Human pulmonary microvascular endothelial cells (HPMECs) were purchased from Promocell (Heidelberg, Germany) and cultured either in T25 flasks, 6-well plates, 8-chamber Lab-Tek, or E-plates L16 (Acea Biosciences, San Diego, CA, USA). .. To improve cell adherence, T25 flasks, 6-well plates, or 8-chamber Lab-Tek slides were coated with attachment factor (Gibco, Waltham, MA, USA).

    Article Title: Plasma MiR-190 is a potential clinical biomarker for acute respiratory distress syndrome in children and its regulatory role in ARDS cell models by targeting KLF15.
    Article Snippet: Background: The present research aimed to investigate the clinical value of plasma miR-190 in children with acute respiratory distress syndrome (ARDS) and the impact of miR-190 on LPS-induced ARDS cell models.. Methods: The plasma miR-190 levels were measured using real-time quantitative reverse transcription PCR (RTqPCR).. LPS-treated human pulmonary microvascular endothelial cells (HPMECs) were established and then transfected with miR-190 mimic, inhibitor, or miR-negative controls.



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    HPMECs were treated with 1 µg/mL of LPS for different time. A Cell viability in HPMECs. B The concentration of TNF-α. C The concentration of IL-6. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001

    Journal: BMC Pediatrics

    Article Title: MiR-483-5p has the potential to serve as a biomarker in perioperative respiratory adverse events in children under general anesthesia

    doi: 10.1186/s12887-025-06483-x

    Figure Lengend Snippet: HPMECs were treated with 1 µg/mL of LPS for different time. A Cell viability in HPMECs. B The concentration of TNF-α. C The concentration of IL-6. n = 3. * P < 0.05. ** P < 0.01. *** P < 0.001

    Article Snippet: Human pulmonary microvascular endothelial cells (HPMECs) were provided by PromoCell (C-12281, Heidelberg, Germany) and cultured in endothelial cell medium (ECM) supplemented with FBS, antibiotics, and endothelial cell growth serum.

    Techniques: Concentration Assay